This study shows the bacteriological investigations of Diarrhoeal diseases among selected children who were between the ages of 0 – 14 years. Stool samples from primary school pupils in Akoko South West Local Government Area in Ondo State, Nigeria were used for this purpose. The organisms were isolated and identified using cultural and biochemical test. Isolates obtained were subjected to some antibiotic susceptibility testing. Out of total number of 120 samples examined only 40 (33.3%) of primary school pupils were found to have Diarrhoea associated with bacteria in which 17 (42.5%), 15 (42.5%), and 8(32.5%) were from 0-4 years, 5-9 years and 10-14 years respectively. The Bacteria species isolated include Escherichia coli were the most predominant microorganism. This is followed by Staphylococcus aureus, Bacillius cereus and Vibro cholerae respectively. As at the time of the study, there was significance difference between male and female gender with the male having high incidence of 55.0%. Among the antimicrobials used sparfloxacin and cefuroxime was most effective against Gram negative and Gram postive bacteria respectively. Also, most of the children tested were found to have common illness with clinical manifestations of diarrhoea followed by dysentery, abdominal pain and fever which may be accompanied with vomiting. Severity of infections varies with individual.
Jones T, McMillan M, Scallen E,. Frenzen P, Cronquist A, Thomas S, et al.: A population-based estimate of the substantial burden of diarrhoea disease in the United States; Network (FoodNet): Population Survey Atlas of Exposures. 2002, 129:9-17
Simiyu S: Water risk factors pre-disposing the under five children to diarrhoea morbidity in Mandera district, Kenya. 2010. East Afr J Public Health, 2010, 7(4):353-60
Ziv T, Heymann D, Azuri J, Leshno M, Cohen D: Assessment of the underestimation of childhood diarrhoea disease burden in Israel. Epidemiol Infect. 2011, 139(9): 1379-87
Rajib D: Exploring intrahousehold factors for diarrhoea diseases: A study in slums of Delhi, India. Journal of Water and Health. 2008, Vol. 06.
Eshete, WA: stepwise regression analysis on under-five diarrhoea morbidity prevalence in Nekemte town, western Ethiopia: maternal care giving and hygiene behavioral determinants. East Afr J Public Health 2008, 5(3):193-8
Mekasha A, Tesfahun A: Determinants of diarrhoea diseases: a community based study in urban south western Ethiopia. East Afr Med J. 2003, 80(2): 77-82
Mukherjee A, Das K, Bhattacharya M, Nozaki T, and Ganguly S: Trend of Entamoeba histolytica infestation in Kolkata. Gut pathog. 2010, 2:12
Luby S, Halder A, Tronchet C, Akhter S, Bhuiya A, Johnston R:.Household characteristics associated with hand washing with soap in rural Bangladesh. Am J Trop Med Hyg. 2009, 81 (5): 882 – 887
Luby S, Halder A, Huda T, Unicomb L, Johnston R: The effect of hand washing at recommended times with water alone and with soap on child diarrhoea in rural Bangladesh: an observational study. PLoS Med.. 2011, 8(6): e10001052
Fink G, Günther I, Hill K: The effect of water and sanitation on child health: evidence from the demographic and health surveys 1986-2007. Int J Epidemiol. 2011, 40(5), 1196-1204
Abdul R, Mutnuri L, Dattatreya P, Mohan D: Assessment of drinking water quality using ICP-MS and microbiological methods in the Bholakpur area, Hyderabad, India. Environ monit assess. 2012, 184(3):1581-92
Ganesh K, Kar S: Health and Environmental sanitation in India: Issues for prioritizing control strategies. Indian J occup Environ Med. 2011, 15(3): 93-96
Roy S, Scallen E, Beach M: The rate of acute gastrointestinal illness in developed countries. J Water Health. 2006, 4 suppl 2:31-69
Bauer S, Kirby W: Antibiotic susceptibility testing of shigellosis in children. American Journal of clinical. Pathology 2004, 45: 70 – 703
Cheesbrough M: District laboratory practice in tropical countries. ECBS edition .Cambridge University Press 2000, 2: 80-85.
Cheesbrough M: District laboratory practice in tropical countries. Part 2. Press syndicate of the university of cambridge publisher. 2000 b, Pg 101-178
Amarenco G: "Bristol Stool Chart : étude prospective et monocentrique de « l’introspection fécale » chez des sujets volontaires" [Bristol Stool Chart: Prospective and monocentric study of 'stools introspection' in healthy subjects]. https://en.m.wikipedia.org/wiki/Bristol_stool_scale.
Progrès en Urologie (in French) 2014, 24 (11): 708–13. doi:10.1016/j.purol.2014.06.008. PMID 25214452.
Alkizim F, Matheka D, Murithi A: Childhood diarrhoea: failing conventional measures, what next? Pan afr medical journal.: 2011, 8:47
Arora D: Text book of Microbiology. Second edition. New York, United Kindom. 2001, Pp. 294- 300.
Gallies R: Lecture notes on Medical Microbiology, 2nd edition Black well Scientific Scientific Publications, Churchill living stone, New York 2004, Pp.114.
Stephen M: Shigellosis in kids and teens. Journal of kids’ Health American Academy of Family Physician 2004, 3: 2-5.
Kotloff K, Winkoff J, Invaoff B, Clemens JD, Swed W: Global burden of Shigella infections. Implication for vaccine development and implementation. 2005, WHO 77 (8): 657 – 659 .
Iwalokun B, Gbence G, Adewale T, Akinsinde K: Shigellocidal properties of three Nigerian medicinal plants (Ocemon gratissimum, Terminalila aviconnoides and Momardica baisamina). Journal of Health and Population Research., 2001. 19 (4): 331-335
Zubairu SM: Paediatric articles on Breast Feeding. Journal for National Health Issue of Muslim Students. The healing message, 2002. Pp 23-25.
Perilla M: Manual for the laboratory identification and antimicrobial testing of bacterial pathogens of public health importance in the developing world. World health organization. Atlanta Georgia U.S.A., 2003. Pp. 121 – 275.
WHO: Manual for laboratory investigations of acute enteric infections WHO 2004,. CDO/83.
Kingston M: Biochemical disturbances in Breastfed infants with gastroenteritis and dehydration. Journal of paediatric, 2003. 82: 109-113.
This work is licensed under a Creative Commons Attribution 4.0 International License.